1 Photon, 2 Color Miniature Head-mounted Fluorescence Microscope
I have spent the last year and a half working with the Gardner-Otchy Lab at Boston University to develop and optimize a two-color adaptation of their FinchScope.
The 1 Photon, 2 Color (1P2C) microscope is the first miniscope to allow for the simultaneous imaging of two spectrally distinct fluorescent reporters in the brains of freely-behaving animals with only one CMOS.
I had the incredible opportunity to present my work at the 49th annual Society for Neuroscience Conference in Chicago, IL on October 23, 2019.
SolidWorks rendering of an assembled 1P2C
The Boston University miniscope team
Answering questions during the poster session
My contributions to this project include: modeling and optimizing the optical path of the microscope, development and integration of the illumination circuit and DAQ in conjunction with William Yen, & assembly and characterization of all 1P2C miniscopes used in neurobiological experiments.
I have also begun the process of updating the documentation on the lab’s GitHub Wiki, found here.
Assembled DAQ box
Fully assembled illumination circuit
Scale image of a 1P2C next to Benny from the Lego Movie